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In some co-culture experiments, as shown in Fig. 1c and d, THP-1-derived macrophages were seeded at different density or co-cultured for different time in 2 × 105/mL.
Cells were seeded at different density in 12 well plates (Corning) containing 19 mm diameter coverslips and incubated at 37°C for 24 hours.
Mtb cultures subjected to multiple-stress for different periods of time were fractionated on a Percoll density gradient and subsets of Mtb population were separated at different density levels at different time periods during multiple-stress treatment (Fig. 5).
For example, the P22 and T7 phages both belong to the family Podoviridae, but have been shown to sediment at different density layers in a CsCl gradient [ 24].
HE staining of sciatic nerve showed normal structure in control group and mild atrophy of axons and diffused structure of myelinated fibers at different density and size as well as demyelination in Mod group.
The study was carried out in a fish farm located in Northern Italy (Ticino basin, Province of Pavia), where several species of sturgeons are reared in raceways system at different density depending on the size of fish.
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There were no significant differences in feed conversion ratios among the fish stocked at different densities (p > 0.05).
Daynard, T. B. & Muldoon, J. F. Plant-to-plant variability of maize plants grown at different densities.
In this paper three design proposals for cities in the U.K., at different densities are used as case studies.
The mechanical behaviour is investigated through oedometric and triaxial tests, performed in either PUR and XPS samples at different densities.
Using the liquid quenching method we generate amorphous carbons at different densities, and subsequently anneal at high temperature.
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