Cell culture media was collected at designated time points for mouse and human VEGF analysis.
At designated time points following sgRNA lentivirus transduction, cells were collected and washed with PBS.
We found that bone mass consistently decreased and the bone microarchitectures continuously deteriorated in male ARKO mice at designated time points.
At designated time points, the samples were rinsed gently with distilled water three times and prepared for mass analysis after collection through centrifugation.
Bromodeoxyuridine (BrdU) (150 mg/kg body weight, dissolved in 0.9% NaCl (Roche, Indianapolis, IN)) was injected intraperitoneally once per day for 2 days at designated time points.
After irradiation, chromosomal aberrations of cells were analyzed at designated time points using G2 phase premature chromosome condensation (G2-PCC) coupled with Giemsa staining and with multiplex fluorescent in situ hybridization (mFISH).
Energy metabolism was evaluated at designated time points throughout the study by performing morning oral glucose tolerance tests and measuring levels of glucose, insulin, glucagon, leptin, ghrelin, adiponectin, resistin, and brain-derived neurotrophic factor (BDNF).
At baseline, and at designated time points during the 8-week study, asthma control, symptoms, and Quality of Life questionnaires (ACQ, ASUI, mini-AQLQ) were assessed and blood was collected for analyses of markers of general health, oxidative stress, and inflammation.
Efficacy: Difference in remission (YMRS (Young et al. 1978) score of ≤12 or equivalent) between lithium and comparatively treated patients at designated time points.
Efficacy: Difference in response (as defined by a decrease in score on any validated mania rating scale of ≥50% from baseline) between lithium and comparatively treated patients (placebo or other anti-manic agent) at designated time points.
The animals were sacrificed with CO2 asphyxiation followed by cervical dislocation at designated time points after tracer administration, and samples of tissues and body fluids were collected for radioactivity measurements and weighing.
