The phrase "at both steps" is correct and usable in written English.
It can be used when referring to two specific stages or phases in a process or procedure.
Example: "We need to ensure that quality control is maintained at both steps of the manufacturing process."
Alternatives: "at each stage" or "in both phases".
(It was experimentally observed that using confidence measures at both steps of the lexical cohesion measure leads to better results than using confidence measures solely in the language model estimation step or in the probability calculation step).
This tool comes into play at both Steps 3 and 7 in our curation workflow diagram.
Our method takes a fully frequentist approach by adopting bootstrap methods at both steps.
We included negative control reactions with bidistillated water in each experiment at both steps of nested PCR.
We detected single-copy orthologs among the five strains using a two-step procedure that incorporates both homology and synteny at both steps.
At both steps, each publication for screening was reviewed by two researchers using pre-defined inclusion criteria (Additional file 2: Table S1).
The mix was incubated in a thermocycler with the following parameters: incubation for 2 minutes at 37°C, 5 minutes at 16°C, both steps repeated 45 times, followed by incubation for 5 minutes at 50°C and 10 minutes at 80°C.
The restriction-ligation mix was incubated in a thermocycler with the following program: 5 minutes at 37°C and 5 minutes at 16°C, both steps repeated 50 times, followed by incubation for 5 minutes at 50°C and 5 minutes at 80°C (trypsin shuffling experiment 1, ts1, Table 2).
Programs were defined with the following steps: incubation for 2 minutes at 37°C and 5 minutes at 16°C, both steps repeated either 25 or 50 times, followed by incubation for 5 minutes at 50°C (final digestion) and then 5 minutes at 80°C (heat inactivation).
After an initial denaturation step of 3 minutes at 94°C, the cycling profile was 20 seconds at 52°C, 40 seconds at 72°C and 30 seconds at 94°C for both steps but the number of cycles was 25 in the outer PCR and 30 in the inner PCR.
At present, both step-down models and outpatient therapies are realistic treatment options for the majority of dysfunctional PD patients, and comparisons between treatments at such different levels of care are needed.
