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The phrase "at ambient temperature" is correct and usable in written English.
You can use it when you are describing something that is at a temperature that is the same as the surrounding environment. For example, "The pie is best served at ambient temperature."
Exact(60)
Rabbit: the loins are poached and served at ambient temperature.
At ambient temperature, n.m.r.
This investigation was performed at ambient temperature.
The columns were operated at ambient temperature.
Chromatography was carried out at ambient temperature.
Experiments were carried out at ambient temperature.
120 and dried at ambient temperature.
Water was freely available at ambient temperature.
All solutions are miscible at ambient temperature.
Length of the steel strand at ambient temperature level T0.
Measurements have been taken at ambient temperature (15°C).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com