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Subjects wore foam earplugs with an NRR attenuation rating of 33 dB and wireless headphones playing pink noise at a volume sufficient to mask any sounds produced by the vibrating plate and the motors.
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One day after seeding, the medium was removed, and the cultures were incubated with 300 mM D-glucosamine in Hank's salt solution (HSS) for 30 min at 37°C in a volume sufficient to cover the monolayer.
To ensure a sufficiently high concentration, the tranexamic acid was diluted only to a volume sufficient to moisten a fairly large wound surface 32: 20 ml moistens at least 1500 cm.
Tissue culture plates were incubated with ECMP coating buffer (PBS with 15 ng/ml Collagen I [C1], 15 ng/ml Collagen III [C3], 15 ng/ml Collagen IV [C4], 50 ng/ml FN, 15 ng/ml VN) overnight at 37° with volume sufficient to coat the surface area of the well.
Ventilation was mechanically controlled at a frequency and tidal volume sufficient for maintaining normocapnia.
At the center of the Sun is the core region, a volume of sufficient temperature and pressure for nuclear fusion to occur.
We observe levels of IFN lambda in hantavirus preparations that are sufficient to activate an ISG response when presented to epithelioid cells at a volume corresponding to less than an MOI = 1.
This approach consists in solving the conservation equations (mass, momentum, energy) averaged in a control volume at a scale sufficient to contain several solid particles in the surrounding gas mixture.
In brief, tissues were harvested directly into a volume of staining solution sufficient to cover the tissue and placed under a house vacuum for 10 min. Staining was carried out at 37°C in the dark for 48 hr.
These have a solid volume fraction sufficient to sterically hinder the gravity-induced phase separation.
Here, we describe a large-scale, electron tomography investigation of S. pombe, including a 3D reconstruction of a complete eukaryotic cell volume at sufficient resolution to show both how many MTs there are in a bundle and their detailed architecture.
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