Exact(4)
The search criteria were set at a stringent level (e-value > E-50) in order to select sequences with a relatively high sequence alignment length and homology to the microarray query sequences.
In addition, many metabolites found to be associated with diabetes at a stringent level of significance in one biofluid were also associated at a nominal level of significance in the other biofluid(s).
These can be based on detecting modest differences in individual genes (gene selection phase) with good power (i.e. 80 90%) at a stringent level of significance (i.e. 0.1 1%) that will help control for multiple comparisons.
The cleaned data were clustered with Vmatch at a stringent level, where the default parameter setting was used, as applied in a number of SNP mining programs [ 30, 45].
Similar(56)
We selected a stringent level of significance because we were doing multiple tests of association.
Results for symptomatic patients are shown thresholded at FWE P < 0.05; presymptomatic differences are shown at a less stringent level of FWE P < 0.1, with effect-maps (difference between adjusted group means as a percentage of the control mean) provided for a fuller characterization of the atrophy pattern.
When pruning the entire data set (containing ~6M SNPs) at a very stringent level of SNP pruning (r 0.001; resulting in a set of nearly independent SNPs), DE estimates were 13, 11, and 15% for GGT, ALT, and AST, respectively.
These genes were required to be significantly differentially expressed in the dsx D comparisons at a more stringent level (q < 0.05) to avoid false positives.
Defense response (GO 0006952, FDR = 0.047) and chitin metabolism (GO 0006030, FDR = 0.032) were the only processes significantly enriched at a more stringent level (FDR < 0.05).
Because of multiple testing, and the fact that QOL measures are considered secondary end points in the trial, the strength of statistical evidence for differences between groups was pre-determined at a more stringent level than the overall trial design of α = 0.05.
Even at a stringent significance level of α =10-8, the power of Cohen's h remained higher than that of OR at α =5 × 10-8 for SNPs with MAF < 0.001 (data not shown).
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