Oocytes (n = 535) were recovered by means of an aspiration pump (set at a flow rate of 10 mL H2O/min), with a disposable 20 G needle attached.
For this purpose, the reference solution was introduced into the ESI source via a T-junction using an Agilent Series 1200 Isocratic Pump (Agilent Technologies, Santa Clara, CA, USA) using a 100 : 1 splitter set at a flow rate of 20 μL/min.
Nitrogen sheath gas was set at a flow rate of 30 L/min, and auxiliary gas was set at a flow rate of 10 L/min.
To perform the DSC analysis, 5 mg of MePEG-PCL diblock copolymer samples was sealed separately in a standard aluminum pan, and purged with pure dry nitrogen gas set at a flow rate of 10 ml/min, the temperature variation was set at 10 °C/min, and the heat flow was recorded from 30 to 200 °C.
Continuous exchange of the bulk solution (dilution or "rinse" experiment) was achieved by means of two Teflon tubes, which were inserted into the cuvette and connected to a multichannel peristaltic pump set at a flow rate of 5 mL/min.
The system was set to run at 25°C at a flow rate of 3 μl/min.
The initial mobile phase composition of 92% solvent A and 8% solvent B was maintained for 2 min. Between 2 and 9 min the percentage of mobile phase B was increased to 35% and then back to initial the mobile phase composition within 0,1 min, with a total time of 14 min. The column was set at a flow rate of 0.2 ml min−1 and a temperature of 36°C.
Nitrogen auxiliary gas was set at a flow rate of 10 L/min and the spray voltage was set to 4.5 kV.
For the other conditions, the liquid nebulizer was set to 45 psi, the nitrogen drying gas was set at a flow rate of 10 L/min with the drying gas and vaporizer temperatures maintained at 300 °C, and the ionization interface was operated in both positive and negative modes.
The oven temperature was set a 200°C and the carrier gas (helium) was set at a total flow rate of 1 ml/min.
