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Triplex-forming oligonucleotides have been used to specifically introduce DNA lesions at a selected target sequences.
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Variants of the psd module with diverse characteristics are useful to fine-tune the stability of a selected target at permissive (darkness) and restrictive conditions (blue light).
To facilitate intraluminal injection at randomly selected target bowel segment, an open abdominal model was used instead of a laparoscopic model.
Sadarm fires a slug of molten metal at the selected target.
This statement is a separate item to remind all health professionals who care for patients with RA that discussing with the patient the reasons for aiming at the selected target, the therapeutic options available and the strategies planned to reach the target is of utmost importance (category IV).
The matrix fine is particularly useful since it allows the direct parallel inspection of the trajectories on a single 2D image, which is not easily feasible on a movie or its projection, and can also be stored as a list of positions and times at which the selected target has been found for future data analysis.
(B ) RT-qPCR validation of RNA-seq at selected target genes.
Notably, in PRC2-depleted mouse embryonic stem cells (mESCs) (Leeb et al., 2010), and differentiated cells (Pasini et al., 2007), PRC1 proteins have been detected at selected target loci, and moreover, global H2AK119u1 levels are similar to those of wild-type (WT) cells (Schoeftner et al., 2006).
Only a limited number of functional studies has been conducted on P. oceanica, mostly looking at the expression of selected target genes e.g., refs 7,8,9,10.
This led to the suggestion that O-GlcNAcylation of Ser75 stabilizes the EZH2 protein and thereby permits effective H3-K27 trimethylation andand repression of selected target genes (Chu et al. 2014).
Each selected target shows at least two high stringency ChIP-chip sites and one ChIP-pet site along its transcript.
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