After treating with MeJA, the transcription of d4h-like was gradually increased, reaching the highest level, about 25-fold high compared with the control at the 36th hr, and then decreased but still at an up-regulated level after 48 and 72 hr (Figure 4b), suggesting that MeJA could induce d4h-like to express.
Functional category enrichment based on Gene Ontology (GO) was performed for all significant aging genes (n = 276) in the microarray at up- or down regulated levels when aging.
Identification of a large number of differentially regulated genes in each germination phase enables the studies to identify biological pathways and functional groups that are transcriptionally up-regulated or down-regulated at a systems level using MapMan and PageMan tools.
Further analyses indicate that 10 of the 17 up-regulated proteins are probably up-regulated at a post-translational level instead of at the level of the protein concentration: 37 proteins that are differentially regulated in response to salt stress were identified by using SYPRO Ruby stain.
GSTD3 was the most highly over-expressed member of this family (FC = 7.8) whereas the three other GSTs were up-regulated at a lower level (FC < 3); GSTe2- 5, GSTe2 and GSTe1.
The mRNA expression level of CfTCTP in oocytes and fertilized eggs kept at a higher level, and was down-regulated from 2-cell embryos to the lowest level in gastrula.
CHOP is a 29-kDa leucine zipper transcription factor that is ubiquitously expressed at a low level and robustly up-regulated in response to various stress conditions [ 25].
In the third set of experiments (set III), we tested Chinese Hamster Ovary cells (CHO AA8) stably expressing the tetracycline-regulated transactivator tTA at a low level, using the same protocol used previously for HEK cells.
But such metabolic regulatory enzymes are frequently multilevel-regulated, especially at a post-translational level.
Monoallelic expression of the five genes is not coordinated at a chromosome-wide level, but rather regulated at individual loci.
Cla018268 was expressed at a relatively low level and down-regulated during ripening.
