The phrase "at a high depth" is correct and usable in written English.
It can be used in contexts discussing measurements, such as in scientific or technical writing, where depth is a relevant factor.
Example: "The research team conducted their experiments at a high depth to ensure accurate results."
Alternatives: "at a significant depth" or "at a considerable depth".
In this study, we used paired-end Illumina sequencing at a high depth of coverage to sequence one V. cholerae isolate from the Dominican Republic, three isolates from Haiti, and three additional V. cholerae isolates.
To better understand the genetic lineage of these tumours we conducted exome sequencing of synchronous multifocal pTa urothelial bladder cancers at a high depth, using multiple samples from three patients.
Estimation of self-relatedness was optimised at a higher depth (5 10).
Samples T50 and T160, in particular, were sequenced at a higher depth than the other samples, yielding more sequencing data.
All of these observations have to be confirmed with the sequence of a larger number of IMPC cases at a higher depth.
We considered "high coverage" monkeys to be a set of 17 monkeys, each with >25× average coverage, including the 16 monkeys initially chosen for high-depth sequencing and an additional monkey sequenced at a higher depth than originally planned.
Among these genes, eight had some proportion of their exonic bases covered at a higher depth (that is, covered at ≥20×) with ACE (MEN1, RB1, TGFBR1, PKP2, KCNQ1, KCNH2, PCSK9, RYR1) and two showed improved coverage with WGS (MEN1, TGFBR1).
Imputation is considered an efficient and cost-effective way to deal with missing data – the alternative being sequencing at a higher depth – but, to be truly accurate, it supposes that the LD spans a long distance, the marker order is correct, the marker density is sufficiently high and that all haplotypes that need to be imputed are captured in the initial dataset [ 54].
An obvious solution to this challenge would be to sequence the genome at a higher depth of coverage, or to use multiple approaches such as transcriptome sequencing or targeted enrichment in combination with genome skimming (Good, 2011; Weitemier et al., 2014).
As our panel of 100 hotspots only covered 59% of TCGA patients, we examined the panel size needed to cover a majority of patients at a relatively high depth.
Based on this, we analyzed the presence of mutations in 800 900 bp downstream of the transcriptional start site of two highly transcribed genes in pancreas, Elastase1 (Eland and Elastase2 (Ela2a), by next-generation sequencing, which allows large number of mutations to be analyzed at a very high depth (Perez-Duran et al, 2012).
