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Since only ∼3% of the Sox2 molecules in the nucleus are bound to specific DNA sites at a given window of time, it is impossible to infer the spatial distribution of Sox2 enhancer sites simply from fluorescence fluctuations captured by wide-field imaging or from conventional super resolution images of live or fixed cells.

To assess whether there was a clear genome-wide tendency for coordinated expression evolution, we therefore used a permutation approach using total number of significant tests at a given window size (2, 5, 10, and 20) as a test statistic to assess the null hypothesis that there are no more gene clusters where expression is co-evolving than we would expect at random [ 12].

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DAS28-3–ESR DAS28-3 ESRhe nearest time point within a givaluesndow (±2 months for the 6-month atsessmenthewere used.

The posterior probability of inclusion (PPI) for a given window, which is the proportion of samples in which at least one SNP from a given window was included in the model with a non-zero effect, was used for significance testing [ 82].

Posterior probability of inclusion (PPI) for a given window, which is the proportion of samples in which at least one SNP from a given window was included in the model with a non-zero effect, was used for significance testing [ 46].

We then repeated the co-expression analysis for window sizes 2, 5, 10, and 20 and counted the number of windows that had p-values less than 0.001 of the 1000 random sets of a given window size as described in the previous paragraph, i.e. our statistic used to assess significance is the number of windows identified at a given cutoff in our original data (Table 1).

The next schizogony took place simultaneously in the three cultures resulting in 11.6% parasitemia with 5.4% residual schizonts (CQ), in 11.6% parasitemia with 5.1% schizonts (Arte) and in 11.7% parasitemia with 5.0% schizonts at the given window of the sorbitol-induced synchrony.

We therefore adopted a conservative estimate of significance by taking only a subset of pairs containing at most one pair for any given window.

Good adherence was defined as taking at least 90% of prescribed doses at a given time window.

To compare the activities in the same neuronal population in response to two events at sequential time points, we used TetTag bi-transgenic mice in which neuronal activities at a given time window can be persistently labeled.

While cross sectional designs provide important information about prevailing practices at a given time window, the cross sectional method is not well suited for comparisons between antipsychotic treatment groups.

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