Experimental strains (group 5) (BSE, C506M3, 87V), derived from mouse-adapted strains [20], were used at a second passage from TgOvPrP4 mice.
This work is a first attempt at bringing first passage times of stochastic systems into wind engineering by suggesting the use of a first passage time map as a standard analysis tool in experimental wind engineering.
While a first passage of an agent between species is not normally sufficient to confirm strain identity, the similarities identified at first passage in this study are striking.
Virus prevalence was found already at the first passage when a concentration of 0.1% of Triton x-100 was used.
When chondrocytes are expanded in vitro to obtain sufficient cell quantities for implantation, they dedifferentiate, losing their characteristic gene expression profile of collagen II and aggrecan by over an order of magnitude at the first passage, and begin expressing high levels of collagen I [13].
Cytospins were prepared from melanocyte cultures at an early phase (first passage in culture, approximately 7 10-day-old culture), at 3rd passage in culture (3p, about three weeks after primary plating) and at late phase in the 7 10-day-oldcultures (generally five weeks into culture).
Due to elimination of damaged cells, populations of cultured cells seem to become "younger", at least at first passages.
The mice were inoculated by both intra-cerebral and intra-peritoneal routes, from a bovine thalamus sample at first passage and from pools of brains from C57Bl/6 or SJL mice at second passage.
One subgroup showed the histopathological features seen in H-BSE at first passage, and especially a low amount of PrPd which was exclusively detected, by Congo red staining, as amyloid plaques (Figure 3A1, A2, C, E1, E2).
At the first passage, cells are collected and retrovirally transduced.
