Sentence examples for at a defined locus from inspiring English sources
This technique can be used for a wide range of applications, such as structure-function analysis, protein localization and purification, genetic screens or generation of single copy transgenes at a defined locus in the genome.
The developed transformation system yields strains with an additional gene at a defined locus that are prototrophic and ostensibly isogenic compared to their parental strain.
In this study, we expressed the Saccharomyces cerevisiae I- SceI meganuclease to generate double-strand breaks at a defined locus in the Trichoderma reesei genome.
Schematic depiction of the domain organization of CYK-4, the variants tested, and the method for single copy transgene integration at a defined locus.
Our data indicate that DSB mediated by I- SceI in T. reesei improves the efficiency of transformation and the stability of the transformants and promotes gene targeting at a defined locus.
In eukaryotes, these regions consist of short (10 to 100 bp, most commonly 15 bp) sequences of DNA repeated end-to-end at a defined locus in the genome.
However, random mutagenesis, used in TILLING approaches, provides only a limited density of mutational events at a defined target locus.
A genetic engineering toolbox for A. niger is presented, which facilitates the generation of strains carrying heterologous expression cassettes at a defined genetic locus.
Most techniques aimed at detecting mutational events within a gene, such as digital droplet PCR or SNV array technologies, detect specific base-pair substitutions or frameshift mutations at a defined genomic locus rather than across the entire gene length.
To monitor SPD-5 behavior, we generated a GFP fusion under endogenous regulatory sequences by transposon-mediated insertion at a defined chromosomal locus (MosSCI).
Our results demonstrate that tetraploid embryo complementation by F3 hprt negative ES cells facilitates the generation of transgenic mouse embryos containing a single copy gene at a defined genomic locus.
