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While the monotremes are indeed warm-blooded, they like to play it cool: five degrees cooler than us at a chilled out 32C.
Each move went his way, and so the Cardinals took the first game of the 107th World Series, 3-2, in front of 46,406 at a chilled Busch Stadium.
To start, Dr. Eigler and Dr. Schweizer sprayed a beam of xenon atoms at a chilled crystal of nickel, leaving a random pattern of the atoms on the surface.
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The pellet was fixed for 1 hour, gently scooped off the bottom of the tube and further fixed overnight (16 to 18 hours) at 4°C with a chilled (4°C) fixative containing 3% glutaraldehyde, 4% paraformadehyde, 4% polyvinylpyrrolidone (MW 10,000), 4% sucrose and 2 mM CaCl2, prepared in 0.1 M cacodylate buffer with a final pH of 7.4.
But you can drop in at other times for a chilled chai or a bittersweet hot chocolate.
Freedman's would have been the favorite restaurant of your late Uncle Morris, the guy who had a drawer full of cuff links, a favorite room at the Sands, and a chilled seltzer gun in the fridge.
The heart tissue was washed three times with the isolation buffer to remove any residual blood and homogenized at 500 rpm with a chilled Teflon pestle in a glass cylinder with 10 strokes.
For each immunoprecipitation 1 mg of nuclear extract was diluted 1 1 with dilution buffer (1 mM EDTA, 1 mM DTT, 1 mM PMSF, complete 1× protease inhibitor cocktail, 10% glycerol) to lower the salt concentration and pre-cleared with Protein A agarose beads (Sigma) for 3 hours of incubation on a rocker; samples were recovered by centrifuging for 2 min at 2000 rpm in a chilled micro centrifuge.
Temporal bones housing the cochleae were removed, opened, and placed in a chilled (at 4°C) physiological salt solution.
Immediately following elution, individual oligomeric populations were collected and individually loaded onto PVDF membranes equilibrated in 20 mM Tris at pH 7.4 using a chilled Minifold I dot-blot system (S&S).
But for many at the typical workplace, it's a chilled glass of beer at a local watering hole.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com