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In order to assure the specificity of the study results, the results of MDD patients will be compared to those of healthy controls.
A parallel reaction was set up for every experiment with the inhibitor of respective complexes to assure the specificity of the reaction.
All real-time PCR runs included a melting curve analysis to assure the specificity of the product with each PCR reaction.
It will be desirable to determine the genomic sequence across the wider MHC region in order to assure the specificity of each MHC locus in the bear genome.
To assure the specificity of our results, C4HD tumors growing in the presence and absence of MPA were included in the TMAs as positive and negative controls, respectively.
To assure the specificity of our results, C4HD tumors from the model of mammary carcinogenesis induced by progestins [ 20] were included in the TMAs.
Similar(51)
This assures the specificity of analysis in the absence of chromatographic separation.
Thus, only the PrP resistant form to PK digestion (the PrP form usually considered to be detected by western blot method) remains detectable, assuring the specificity of the detection.
Polymerase chain reaction used consisted of 3 min at 95°C, followed by 30 cycles at 95°C for 15 s and 60°C for 1 min. To assure the amplicon specificity for each primer set, the PCR products were then subjected to a melting curve analysis.
To assure the amplicon specificity of each primer set, the PCR products were then subjected to a melting curve analysis.
This approach is simple but has the drawback of not taking into account the specificity of inter-view estimation and cannot assure the best performance.
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