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The phrase "assume not" is correct and usable in written English.
It can be used to advise someone against making assumptions or to encourage critical thinking.
Example: "In discussions about sensitive topics, it is important to assume not that everyone shares the same perspective."
Alternatives: "do not assume" or "refrain from assuming".
Exact(53)
Assume not.
I assume not.
NICK PAUMGARTEN: I assume not.
One has to assume not.
(The owners, I have to assume, not the dogs themselves).
I, and the rest of the American electorate, assume not.
Similar(6)
The majority of these are assumed not to survive.
The added material is assumed not to be prestressed.
I'm assuming not.
Infectious persons are assumed not to travel.
Microsatellites are assumed not to be subject to selection.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com