βB2-N thus resembles the ancestral prototype of the βγ-crystallin superfamily as it self-associates in solution to form a dimer with an essentially identical domain interface as that between the N and C domains in βγ-crystallins, but without the benefit of a covalent linker.
We have trained and validated this method on three model protein complexes which are not known to form higher order oligomers, and one which has a tendency to self-associate in solution in a concentration dependent manner.
In these complexes, the intermolecular interactions are much weaker in the solid state and they appear not to be significantly associated in solution.
However, no such change in the mobility was observed, suggesting that if Ku DNA complexes do associate in solution, such junctions are not stable during electrophoresis.
Analytical ultracentrifugation (AUC) demonstrated that all four catalytic domains self-associate in solution, whereas a site-directed mutant of SLK (Q185P), which is monomeric in solution, showed that dimerization is required for autophosphorylation.
Cations that possess acidic protons are capable of undergoing significant hydrogen bonding to the halogermanate anion in the solid state, and NMR evidence suggests the cations are associated in solution, albeit more strongly in the GeIV than GeII systems.
It has been demonstrated that DNA:mC MTase M. AquI comprises two independent polypeptides corresponding to the catalytic domain and the TRD, which associate in solution to form a functional enzyme [ 37].
In summary, these first experiments with all five purified components demonstrate that dynein-Lis1 and Bik1-Bim1-Kip2 eacomplexesomplexes that are sufficiently stable to co-elute by size-exclusion chromatography.> Given that the dynein-Lis1 and Bik1-Bim1-Kip2 sub-complexes did not stably associate in solution, we hypothesized that these two assemblies might interact dynamically on the microtubule.
However, despite these differences, these two oil fractions are similar in that they have a high molecular weight, aggregate or associate in solutions, and have a limited solubility in crude oil.
It was explained by the formation of various-type associates in the solution and on the oxide surface.
