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During replication, the primase is a constitutive component of the replisome, the protein complex associated with the replication fork [3].
The resistance level of a vPhenotype is based on observed phenotypic fold-changes (FC) in IC50 associated with the replication of a virus with a given sequence.
The greater diversity at these sites could potentially indicate higher rates of mutation associated with the replication of genome ends.
We have previously shown that Tipin is associated with the replication fork and is required for fork stability and fork restart (Errico et al, 2007).
An alternative model is that factors associated with the replication machinery facilitate cohesin ring opening upon fork passage, and its reclosure in the wake of the polymerase.
Because BAZ1B is associated with the replication of pericentric heterochromatin in somatic cells (Bozhenok et al., 2002), we tested whether Baz1b deletion disrupts heterochromatin formation during meiosis.
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We were particularly interested in ELYS because we had also identified it in a screen for factors associating with the replication licensing proteins Mcm2-7 on chromatin (see below).
Epigenetic maintenance factors are also associated with the DNA replication machinery5 and therefore DNA replication represents a similar opportunity for deregulation of the epigenome.
Alternatively, since AR is associated with the DNA replication machinery ([ 75]; S. Murthy, U. Bai and G.P. Reddy; manuscript in preparation), and since telomere DNA replication utilizes the DNA replication machinery [ 82], it is conceivable that AR inactivation may compromise the efficiency of the DNA replication machinery during the replication of fragile sites in telomeres.
Moreover, the PB2 subunit is associated with the efficient replication of cold adapted influenza virus [24], [25], [26].
In control conditions, full BrdU incorporation was observed at 10 min after pI division and the punctuate incorporation of BrdU associated with the late replication of heterochromatin was observed at 60 min after pI division (Fig 5A, neu>YFP).
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