Therefore, it is concluded that the diffusion of (FH nF− anions is faster than that of each cation in every melt and that a part of both cation and anion in each melt may associate by interaction between both ions.
Bcl-2 family proteins detected on both normal and cancer cells mitochondria (Fig. 6) remind present after alcaline washes (not shown) indicating that they are not associated by electrostatic interaction with the mitochondrial membranes and are not coming from residual cytosol or endoplasmic reticulum.
Since the presence of 100 µg/ml ethidium bromide does not interrupt the coimmunoprecipitation of MDC1 and TonEBP/OREBP-V5, we conclude that MDC1 and TonEBP/OREBP are physically associated by protein-protein interaction.
These two chains are associated by non-covalent interactions forming a macromolecular complex where three distinctive regions are recognized: an intracellular, a transmembrane and an extracellular region, the latter comprising about two thirds of the molecule.
Figure 3B further underlines, in the case of E. coli, that several of these gene products are known to be associated by protein-protein interactions as published in STRING database 9.05 [ 23] while being involved in purine salvage [ 24].
The polypeptide can be further processed into an N-terminal fragment of 33.4 kDa (named p34 or p37, residues 1-311) and a C-terminal fragment of 54.8 kDa (named p55 or p58, comprising residues 320-821), both parts stay associated by non-covalent interactions.
Although the interactions among VP664, VP51C and other structural proteins are not yet completely known, VP664 and VP51C may associate by direct interaction or else by means of some other proteins/factors.
Although we did not determine whether Sox2 is able to self-associate by direct interaction, our studies do indicate that Sox2 uses several domains to promote its self-association (Figure 6D).
Treatment of membranes with high salt or sodium carbonate pH 11.5 releases peripheral membrane proteins that are associated by ionic or hydrophobic interactions respectively, whereas integral membrane proteins are only solubilized by detergents (Helenius and Simons, 1975; Fujiki et al., 1982).
In order to list all the partners of a protein, interactions are associated by default to the accession number of the human protein.
Compared with our previously developed dynamic network model for single-species network construction [ 16], it should be noted that in addition to the single-species protein-protein interaction captured by the term associated with the interaction ability b k m, interspecies interactions are captured by the term associated with the interaction ability c k j.
