However, under these conditions RNA binding proteins are free to dissociate from their endogenous RNA targets and re-associate with higher affinity binding sites, thus there is great risk of false discovery [ 6].
Energy logos for AncSR1 (left) and AncSR1+RH (right) show the effects of nucleotide states on binding energy relative to the average across all REs tested; states with ΔΔGdissociation > 0 are associated with higher affinity binding.
Because the individual intein segments associate with high affinity, their assembly can be co-opted for purification.
The existence of cell surface heparanase receptor(s) is supported by binding experiment, reinforcing the notion that while HSPGs serve as low affinity, high abundant binding sites, heparanase also associates with high affinity, low abundant cell surface receptor(s) [67].
The decrease in the levels of GSH following exposure to arsenic has been found to be associated with high affinity of arsenic with GSH [ 51].
Specifically, quetiapine is associated with high affinity at the H1 receptor where it acts as an antagonist ; this mechanism is associated with sedation in both animal models and human studies.
Much of the total cellular copper is associated with high affinity binding proteins, and what is considered labile copper is effectively buffered by a plethora of cellular ligands that minimize free copper ions.
Human Crm1 (top) associates with Rev-RRE with higher affinity than murine Crm1 (bottom) due to species-specific residues that form the dimer interface (gold).
The cleaved C-terminal region of eIF4G could be associated with eIF3 and eFI4A, binds with higher affinity to viral mRNAs and facilitates translation of its own protein [26], [28].
For instance, those ligands with high activity at the α1 are associated with stronger hypnotic effects, whereas those with higher affinity for GABAA receptors containing α2 and/or α3 subunits have good anti-anxiety activity.
