Sentence examples for assists isolation from inspiring English sources

Chlorination treatment being a chemical treatment is a well-established treatment which assists isolation of high quality pure cellulose fibres by bleaching and delignifies the cellulose material, while alkali treatment dissolves the wax, pectin and hemicellulose ensuring efficient isolation of cellulose microfibres.

Utilizing techniques such as MAINE-FAIRE (micrococcal nuclease-assisted isolation of nucleosomal elements and formaldehyde assisted isolation of regulatory elements) or chromatin immunoprecipitation, both coupled to high throughput sequencing approaches, reveal a relative depletion of nucleosomes over poly dA.dT tracts of increasing length [ 2, 4– 6].

In a previous study, we analyzed the nucleosome landscape of the P. falciparum genome during the asexual cycle by a combination of micrococcal nuclease (MNase) assisted isolation of nucleosome-bound DNA (MAINE) and formaldehyde-assisted isolation of protein-free DNA (FAIRE), coupled to next-generation sequencing [ 18].

More recently, flow cytometry assisted isolation method based on DNA contents and mitochondrial propensity have been developed to separate non-tumorigenic ESC sub-lineages [ 5].

(A ) DNA accessibility at FIL and LFY loci in the context of chromatin assayed by Formaldehyde Assisted Isolation of Regulatory Elements (FAIRE) in response to auxin treatment in wild-type (WT) and brm-3 syd-5 inflorescences.

CGI: CpG island; DMSO: Dimethyl sulfoxide; FAIRE: Formaldehyde Assisted Isolation of Regulatory Elements; HSC: Hematopoietic stem cell; HSPCs: Hematopoietic stem and progenitor cells; H3K9me2: Histone H3 Lysine 9 di-methylation.

To assess the accessibility of the MP bound regions at the FIL and LFY loci in inflorescences, we employed Formaldehyde Assisted Isolation of Regulatory Elements (FAIRE), a method that enriches accessible (nucleosome depleted) genomic DNA from crosslinked chromatin after phenol/chloroform extraction (Simon et al., 2012).

To investigate this, we examined the consequences of G9a/GLP-dependent H3K9me2 patterning on chromatin structure in HSPCs using FAIRE-seq (Formaldehyde Assisted Isolation of Regulatory Elements Sequencing) [ 13] to map accessible chromatin in CD34+ HSPCs treated with UNC0638 or dimethyl sulfoxide (DMSO) control (see Methods and Additional file 1: Table S1).

AW assisted with study design, assisted with study coordination, assisted with virus isolation, and assisted with RT-PCR.

We thank Drs Eli Pikarsky and Ariel Revel for assisting with isolation of human embryonic liver tissues and Ms Shelly Tannenbaum for editing the manuscript.

The same procedure may assist in the isolation of ts mutants in other genes in Dictyostelium and perhaps can be adapted for use in other organisms.