Sentence examples for assisting load from inspiring English sources

Runs under assisting load conditions for DmK-5AA and DmK-6AA were collected using an improved force clamp (Milic et al., 2014).

However, the finding that assisting load fails to appreciably increase the WT velocity suggests that the rate of rear-head release by WT kinesin must be substantially higher than the rate-limiting step(s) of the mechanochemical cycle.

We strongly favor the former possibility, because it also helps to explain the observed decrease in run length under assisting load, as well as the force dependence of exponential fits to the run length data.

(A ) Mean run lengths (mean ± SE; N = 84 210) under moderate assisting load (+4 pN), in the presence of nucleotide analogs (green), 100 mM potassium chloride (KCl; purple), or 100 mM potassium phosphate (KPi; orange).

These data are consistent with the explanation that inter-head tension, which can promote rear-head release, is effectively abolished by extending the NL past its WT length, but that the reduced tension can be restored by applying assisting load, which places differential stress on the rear head.

Put another way, if load-dependent rate k3 was instead to correspond to tethered-head binding, then an increase in k3 under assisting load would increase the likelihood of completing a step, and thereby increase run lengths in this force regime, contrary to observation.

Under assisting loads, only the HsK-CL construct could be sped up beyond WT velocities.

Data were collected under the same conditions as Figure 3. DmK-WT velocity was not affected by assisting loads, but the velocities of all mutant constructs could be increased by larger assisting loads.

In contrast to DmK-WT, which is not sped up by assisting loads, the reduction in unloaded velocity produced by NL extension could be recovered by applying larger assisting loads (∼20 pN).

However, for the mutant constructs, we are able to detect a second force-dependent contribution that is manifested as increased velocity under assisting loads.

In the presence of forces applied either against (hindering loads) or along (assisting loads) the direction of kinesin motility, the differences in processivity among constructs with differing NL lengths were substantially more pronounced under hindering forces than under assisting forces.