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The citric acid assisted sample is found to be superior in terms of discharge capacity, capacity retention rate and also in thermal stability to that of sample prepared with glycine as chelating agent.
NS and ST assisted sample collection and data analysis.
SS assisted sample collection and contributed to the writing of the manuscript.
Samples were proteolysed using the 'Filter Assisted Sample Preparation' (FASP) method [ 34].
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This is not surprising given the high level of selfing in S. l. cerasiforme and the pattern of genetic structure uncovered in our sample, both factors that favor the marker assisted sampling strategies over pure random strategies [ 10, 11].
In a large review of core and VAM needle biopsies, DCIS was found at surgery following a biopsy result of ADH in 41% of core biopsies and only 15% of vacuum assisted samples.
This finding may be surprising given that the mating system of M. truncatula (high levels of selfing) and the patterns of regional subdivision uncovered in our sample are factors that a priori favor marker assisted sampling strategies over pure random sampling to capture efficiently variation [ 12].
For both digests, STAGE tip-assisted sample purification was achieved essentially as described (Rappsilber et al., 2007).
It has the advantage of allowing usual-care allocation of clients and would provide an estimate of the ICC which would assist sample size planning in a larger-scale subsequent trial, if judged to be feasible and valuable.
A list of categories was created, using existing literature and expert advice (from a clinical and lay advisory group) to assist sampling.
The authors also thank the HIVNET 012 study team, and the women and infants in these studies, and the staff in Uganda and JHU who assisted with sample processing.
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