ASVCR: Performed the study and prepared the manuscript; SGR: Provided assistance in the investigation on cell lines; PPB: Provided cell lines and supervised the study on cell lines; ARR: Supervised the work, provided the grants for the study, evaluated the data, corrected the manuscript for publication and coordinated the study.
We thank Dr. K.M. Frick (Yale University, New Haven, CT) for the help provided in setting up the assay for measuring GAD activity and Elisabete Carvalho for assistance in the preparation of cell cultures.
The pre-publication history for this paper can be accessed here: http://www.biomedcentral.com/1471-2407/10/481/prepub We are deeply grateful to E.G. Konstantakou, Ph.D. candidate (Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Panepistimiopolis, Zografou, Athens, Greece) for her valuable assistance in the maintenance of cell cultures and scientific advice.
Prof. T. Suzuki for the technical assistance in the treatment of the cell microarray chips.
We would like to acknowledge the substantial contribution of Angelika Ackermann in the establishment of all of the methods used in this study and her invaluable assistance in cell culture, in the immunofluorescence stainings, and in performing the Western immunoblots.
Crucially, the number of misfolded proteins requiring assistance from the cell's protein quality-control pathways is increased in aneuploid cells.
The authors thank Richard Rossoll, M.S., Dartmouth Medical School, Deena Ratner, B.S., University of Pittsburgh, Irma Rodriguez, Brown University, and Jessica Ingersoll, M.S., Emory University, for excellent technical assistance in the preparation of samples, cells and virus stocks.
The authors would like to thank Dr Intawat Nookeaw (Chalmers University, Sweden) for his help in modeling and all the staff at the Algal Technology Laboratory at King Mongkut's University of Technology Thonburi KMUTTT) for their assistance in Spirulina cell cultivation.
