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They are also very useful tools to assist sequence assembly in whole de novo genome sequencing projects [ 1- 3].
Physical maps built from large-insert BAC clones and anchored to linkage maps assist sequence assembly in whole genome shotgun (WGS) sequencing projects [ 1], enable positional cloning of genes/QTLs and structural studies on gene families [ 2, 3], and facilitate the isolation of homologous genes from model plants in heterozygous or polyploid species [ 4].
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Assisting sequence assembly, e.g. identifying breakpoints and duplicated repeats.
Homology models of nAChR subunits were created by using the Orchestrar suite of programs as implemented in SYBYL-X, 38 which essentially is an implementation of Blundell's structure assisted sequencing approach, also known as threading.
The use of multiple technologies to supplement genome sequencing, such as microarray analyses or Sanger sequencing, will assist until sequencing technologies and software for variant analysis overcome these challenges.
YJ and CG assisted with sequencing, sequence analysis and annotation.
In order to assist comprehensive sequence analysis, reliable multisequence alignments were generated and annotated either by an automated pattern search or by information extracted from GenBank (25).
The best one employed a double-Venus, quadruple-Earth, Jupiter Saturn–Jupiter gravity-assist sequence.
We would like to thank Shelby Bidwell for uploading the 454 sequences to Genebank and assisting in sequence assembly, Cook Ly for assistance with preparing figures, and Mark Sorrells for providing comments on the manuscript.
performed cDNA cloning of the KIF27 isoforms and performed sequence analysis J.L.C. characterized and analyzed the cDNA library S.K. performed Northern analysis W.J.H. provided hamster tissues and assisted in sequence analysis S.A.K. assisted in sequence analysis S.C.W. designed the project, performed cDNA synthesis and sequence analysis All authors read and approved the manuscript.
Historically, physical three dimensional (3D) models built by hand provided 3D physical representations of the project to assist in sequencing, visualization, and planning of critical construction activities.
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