Quantification was performed by raster electron microscopy and computer assisted image analysis.
Using zymography and computer assisted image analysis, we have measured the levels of type IV collagenases in biopsies from normal breast, and benign and malignant breast disease.
Total lesion area or area of positive staining was determined for each section/aorta, respectively, using computer assisted image analysis NIS-elements/Lucia GG software, Nikon UK).
The number of cells was estimated by optical fractionator probe using a computer assisted image analysis system with a Zeiss Axioplan 2 microscope connected to a digital camera.
The slices were photographed and infarct size was calculated using automatic computer assisted image analysis software (Clemex, Quebec, Canada) as described before [ 29].
Quantitative determination of density of the [S]klk1b26 protein and [S]GAPDH protein bands was performed by computer assisted image analysis of the autoradiograms.
The images were analyzed using a computer assisted image analyzer system consisting of a microscope (Olympus CX-41 Tokyo, Japan) equipped with a high-resolution video camera (Olympus DP21, Japan).
This negates the need for the operator to use computer assisted image registration methods to evaluate repeated images of the same bone regions in either short term follow up or longer term prospective studies [ 54].
By software assisted image analysis of 2D-DIGE gels, 1505 spots could be matched, i.e., corresponding spots of the three replicates were assigned in a supervised manner, and the intensity of all matched spots was quantified.
Colonies with more than 50 cells were counted using digital images of Petri dishes containing fixed and stained clones, associated with a computer assisted image analysis system (Samba Technologies, Meylan, France) as previously described (Biston et al, 2003), which allows to avoid subjective bias in the procedure.
