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Specific primers were designed to PCR upstream fragments of duf with BamHI or EcoRI overhangs to assist cloning.
FPC-based physical maps were originally made to assist in clone-by-clone sequencing by identifying minimal tiling paths; indeed, the maize FPC map was used for this purpose as recently as 2009 (Schnable et al. 2009).
It is hoped that advanced tools of genomics will assist in cloning useful genes from 1RS.
Although it is premature to suggest that the nine genes are candidate genes for these QTLs, the data indicate that gene expression profiling will be useful to assist in cloning of genes from QTLs.
Markers and experimental plasmids for transgenics, i.e., pMH86 (dpy-20, pPD118.33 (Pmyo-2:: gfp): The pJK590 plasmid (P lag-2) (Blelloch et al. 1999) uses the 3-kb sequence upstream of the lag-2 start codon (5′ tttttaaattctcat caaatttgccttt 3′) and contains a mutated BsmI site within the promoter to assist in cloning.
Research gained in cloning a cheetah would assist in furthering cloning technology and, if it incorporates artificial genetic manipulation in an attempt to expand genetic variation in cheetah populations, it would advance gene therapy technologies, benefitting humans and other species.
Around this time, Clonaid's subsidiary BioFusion Tech claimed to have in possession a cell fusion device that assisted the cloning of human embryos.
assisted in cloning and the generation of stable cell lines.
MWW participated in the rEpCAM signaling experiments and assisted in cloning many of the constructs used in the study.
Experiments were performed by CBL, TN, RAH and VB-V while MFJ and DKG trained CBL and TN and assisted in cloning.
To assist in subsequent cloning steps, BamHI and EcoRI restriction sites were incorporated at the 5'- and 3'-end of all completed constructs.
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