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Tubes and cupules of test glucose fermentation (GLU) and Phenyl-acetate assimilation test (PAC) were filled with the suspension.
Incubation was extended to 48 h if the succinate assimilation test was negative, as indicated by the manufacturer's instructions.
These tests include 19 carbohydrate assimilation test strains along with a negative control and are based on turbidity measurements.
The phenotypic identification of the isolates of C parapsilosis from the blood and hands of HCWs was done by the morphology of colonies, germ tube test, chromogenic medium (CHROMagar Microbiology, Paris, France) and carbon assimilation test (ID 32C; bioMérieux, Marcy l'Etoile, France).
Discrimination between C. albicans and C. dubliniensis was investigated by analyses of germ tube formation in calf serum at 37°C for 3 h, degree of chlamydospore production on cornmeal agar supplemented with 1%tween-80tween-80 at 45°C on SDA, colony morphology at Staib agar, and xylose assimilation test.
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The three strains wild, M1 and M5 were positive for biochemical assimilation tests done with glucose, arabinose, mannose, N-acetyl glucoseamine, maltose, malate and urease.
Of the 122 germ tube positive isolates those were subjected to sugar assimilation tests using xylose and trehalose, to differentiate C. albicans from C. dubliniensis, xylose was assimilated by 108 isolates, trehalose by 114, and xylose and trehalose by 106 isolates.
For routine identification of Candida isolates, conventional methods including germ tube formation, chlamydospore formation and sugar assimilation tests are universally accepted [3].
For convenient identification of Candida spp. germ tube and chlamydospore formation as well as sugar assimilation tests, have been used for a long time [20].
This was confirmed by carbohydrate assimilation tests (Table 2) and by sequencing the 5.8S rRNA gene.
Dextrose, sucrose, and raffinose assimilation tests were performed in triplicate by using yeast nitrogen base medium.
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