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Treatment group assignments were determined using a dynamic randomization algorithm within the interactive voice response system.
Taxonomic assignments were determined using the Ribosomal Database Project (RDP) website classifier.
Pathway assignments were determined using the Kyoto Encyclopedia of Genes and Genomes pathway database (KEGG) [ 41] using a BLASTx threshold cutoff of 10-5.
Orthology assignments were determined using multiz alignment information [ 19], the "chains and nets" data from the UCSC Human Genome Browser mysql tables [ 20].
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Assignment was determined using Steiner's ANB angle according to the following degree of severity: class I (2° to 4°), class II (5° to 10°), and class III (−3° to −6°).
The assignment was determined using a stability cutoff of 15% and a p-value cutoff of 0.9999 (p-values were essentially ignored).
Group assignment was determined using the Hospital Anxiety and Depression Scale (HADS),[ 37] which includes subscale scores for anxiety and depression.
Treatment assignment was determined using an interactive voice/web response system and was stratified by HbA1c at screening [<8.5%6969 mmol/mol) or ≥8.5%6969 mmol/mol)].
Base assignment and quality scores were determined using Phred software [ 55, 56].
Sequence-specific resonance assignments for all labeled proteins were determined using triple-resonance NMR experiments from the Varian standard bio pack pulse sequence library.
The peak intensities were determined using Sparky with assignments previously determined for PKA-C.
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