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Protists comprise a large and sometimes unwieldy assemblage, and assignments of species to particular taxa change as new genomic approaches, biochemical techniques, and electron microscopy studies provide more details on the affinities of various species.
Moreover, considering the large number of species we have observed and how close their Raman shifts are, even small uncertainties in the shifts determined from ab initio calculations make it difficult to make definite assignments of species stoichiometry.
Note that these include our interpretation of some of the assignments of species to groups that are not explicit such as some assignments to the woodland groups or allocation to deciduous versus evergreen.
Conclusions about the generic assignments of species were based on conchological and anatomical (where possible) features.
Information from several genes might also be required to identify cases of recombination that may distort the assignments of species to clusters of single gene sequences.
The at least six biome shifts among the 27 species of Coccinia analyzed here may be an underestimate because of our assignments of species into just three broad biome types, semi-arid habitats, woodland, and forest.
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Here we show that the higher taxonomic classification of species (i.e., the assignment of species to phylum, class, order, family, and genus) follows a consistent and predictable pattern from which the total number of species in a taxonomic group can be estimated.
When modeling food webs, choices must be made regarding the level of complexity for the web, assignment of species to trophic guilds, selection of representative species for guilds, use of average diets, the characterization of variation among individuals or guild members within a web, and the spatial and temporal scales/dynamics of webs.
The assignment of species and subspecies (and potential for determining geographic provenance and female flight ability) with the COI barcode region was compared against the commonly employed 'NB system' [24].
In these latter cases, either the independent analyses failed to resolve the highly similar derived members of the SFG rickettsiae (R. rickettsii, R. conorii, R. sibirica, R. africae), for which the assignment of species names has received recent criticism [82], or failed to place R. canadensis as basal to the TG, TRG and SFG rickettsiae.
The assignment of species to families is based on [ 64] and the assignment of sexual systems is based on [ 65] or where sufficient information is present.
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