Sentence examples for assignments of residues from inspiring English sources
Only the assignments of residues with missing NH signals in holo protein are labeled.
In RNA helices, a similar pattern of NOEs from guanine H1 to the 3′ H1′ on the same strand and the 3′ H1′ on the opposite strand is present and was used to confirm H1′ assignments of residues such as U6, A38, U14, and G29, near GC and GU pairs in the 39-mer.
On the basis of the 13C resonance assignment of residues A and B, the interatomic distances from each C1 to the other carbon nuclei were compared by measuring the 2D spectra recorded with longer mixing times (5.12 20.48 ms).
There is also disagreement with regard to the assignment of residues within the A3G C-CDA that participate in interactions with the substrate DNA.
Most certainly, the multiple exchange processes do complicate assignment of residues to distinct exchange events.
The connection domain H361Y nudge mutation allows further assignment of residues located at the RH interface.
Assignment of residues for nondetect values depended on the commodity: if there was at least one detection, half the limit of detection was assigned; if no As values were detected, zero values were assigned.
Finally, studies on G85R and A4V apoSOD12Shaveve provided confidence in our assignment of residues to particular exchange events, since reporter residues of a particular process in the WT protein that is subsequently abolished via mutation no longer show evidence of conformational exchange.
Additionally, based on the similarity measure used, the assignment of residue-residue equivalences for a structural superposition may differ [23].
Small chemical shift changes were observed only for those residues in the immediate vicinity of the mutation site, and assignments of these residues were again verified by sequential cross-peaks in three-dimensional (3D) HMQC-NOE-HMQC spectra, described below.
