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In order to determine whether the above mentioned incongruent cases were due to the low resolution of the trees or they indicated wrong assignments of clusters of orthologs, detailed analyses of Ycl1 and Ycl2 HKs, Pho and 872 RRs and HKs, and Eta and Kin RRs and HKs were carried out.
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Anatomical location of significant clusters was determined from comparison with a canonical T1-weighted image in MNI space and by automated assignment of clusters to Brodmann areas and brain regions in MNI space (http://www.fil.ion.ucl.ac.uk/spm/ext/ MSU).ac.uk/spm/ext/ MSU
The assignment of clusters to the early visual areas was based on anatomical proximity.
Due to incomplete and dispersed clustering, further manual assignment of clusters and thus repeat phylogeny was necessary.
Although clusters implementing the Pilot Programme were not selected randomly, due to the operational requirements of starting a pilot, scale-up in 2014 includes random assignment of clusters to generate more robust comparisons.
Assignment of Clusters of Orthologous Groups of proteins (COGs) of predicted gene products was carried out by the BLASTP analysis against the COG reference data set (http://www.ncbi.nlm.nih.gov/COG/).nih.gov/COG/
Assignment of Clusters of Orthologous Groups of proteins (COGs) of predicted gene products was carried out by BLASTP analysis against the COG reference data set (http://www.ncbi.nlm.nih.gov/COG/).nih.gov/COG/
Comparison of the tubulysin biosynthetic gene clusters in SBCb004 and An d48 reveals a conserved architecture, allowing the assignment of cluster boundaries.
It should be noted that assignment of cluster membership, as well as the comparison of expression profiles between a tissue of interest and it's complement in the dataset in this study are provisional on the limitations of the sample set used.
In Adaptive Threshold-sensitive Energy Efficient Network (APTEEN), the assignments of the cluster head (CH) are much heavier than other nodes.
Results of the MCL and OrthoMCL cluster analyses consist of an identifier for each cluster and the assignments of proteins to clusters, captured in the classes MclCluster and OrthoMclCluster.
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