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Quantitative and qualitative data on the usefulness of the workshop were collected in the form of pre- and post-workshop surveys and interviews, respectively, and a comparison was made between the two course assignments for further evidence.

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Refer to the parameters of your assignment for further guidelines.

Ambiguous and suspicious well assignments are marked for further investigation and verification.

For further assignments verification, 2D COSY, TOCSY (100 ms mixing time) and NOESY (200 ms mixing time) spectra were obtained, along with C-HSQC and N-HSQC (with the INEPT delay adjusted for short and for long-range couplings) in order to identify NH and N(CH3 2 groups.

Probability of identity and paternity exclusion probability combined across all loci was 5 × 10−6 for the first parent, allowing high-quality assignments (see Richard et al. 2013 for further details on assignment reliability).

The protein set was also searched against COG (http://www.ncbi.nlm.nih.gov/COG/; [64]) and the KEGG (Kyoto encyclopedia of genes and genomes; http://www.genome.jp/kegg/) [65] for further function assignment.

Hence, repeat junction markers will be useful, particularity in polyploidy genomes since they are often genome-specific without the need for further experimental assignment [ 9].

Previously, we have demonstrated that 90%% of PCR-based repeat junction markers derived from Ae. tauschii can be specifically mapped to the respective D chromosomes [ 8, 9] in the hexaploid wheat, with no need for further genomic assignment, demonstrating its potential in development of genome-wide molecular markers for mapping and genetic diversity studies in large and complex genomes [ 10– 10].

Patients are offered a self-help book including background literature for further reading and assignments.

The structure itself can be used as a theoretically modeled structure and also as a starting point for further NOE data assignment in structure determination.

For the literature-collected candidate genes involved in multiple (four or more) CO-enriched pathways we assembled genetic information relevant for further functional analyses: assignment to corresponding biological pathways, genetic variability, and putative presence of polymorphic microRNA (miRNA) target sites (Additional file 6: Table S6).

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