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The consensus classification and nomenclature system for HCV genotype and subtype assignment was established using phylogenetic analysis (3).
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C NMR assignments (C, C H, C H2 and C H3) and H NMR assignments were established with the aid of DEPT-135, HSQC and COSY experiments.
The robustness of the assignment procedure was established using the data obtained in H-N HSQC (Bodenhausen and Ruben, 1980), COCAHA (Dijkstra et al., 1994) and HNCACB (Wittekind and Mueller, 1993) experiments, providing access to backbone and Cβ chemical shifts.
Significance of protein assignment to clusters was established by base composition-preserved randomized pairwise global alignments using the method of Needleman and Wunsch [ 26, 57].
Linkage group assignment and ordering of loci was established based on TMAP software [ 50] that finds the maximum likelihood map using an error-compensating model.
The major strength of our study is that the data was collected in-person, by an interviewer before the disease diagnosis was established and assignment to either the case or control group occurred after the review of tissue pathology reports.
For the high-coverage biofilm samples, microbial diversity was established by taxonomic assignment using reads matching 16S rRNA sequences.
The set of 54 glycans was established by manual assignment of glycan species from all HPAEC-MS /MS) data.
Homology of individual genes was established by reciprocal blast, yielding unambiguous assignment of exons.
A scoring system was established to evaluate confidence in the metabolite assignments.
A committee was established in May 1983, which gave Engineer Harald Møyner the assignment of producing a new report.
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