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A pathologist, blinded to the group assignment, verified all histological diagnoses and counted mast cells on a Giemsa stained tissue section (average of 10 high-power randomly chosen (40X) fields per specimen).
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Predicted novel functional assignments verified by targeted experiments are marked by bold type and underlined.
Results: We have developed a knowledge base of criteria, which replicate expert assessment, allowing more than half of cases to be automatically validated and site assignments verified with a high degree of confidence.
Predicted CDSs were manually annotated individually gene by gene by an international consortium of scientists with expertise on different gene functions on xanthomonads (http://www.reseau-xantho.org/reseau_xantho/). Start codon assignment was verified with special care and suggested automatic annotations were individually expertized to generate the proposed annotations.
For ten of those haplotype phase assignments were verified by cloning and sequencing.
The accuracy of nucleotide assignments was verified by eye for every position.
All reported assignments were verified by automatic and manual interpretation of spectra from Mascot and Proteinlynx search engine and MODi in a blind mode [37], [38].
It is reassuring to note that the structure of the human ortholog of Uso1 has been solved [26] since we produced our prediction data; our repeat assignments were verified and only one repeat was missed by our protocol (data not shown).
Without exception, the subgenome assignments were verified.
The newly obtained sequences were compared with known sarcopterygian Hox genes, and all the assignments were verified.
All assignments were verified by their distinct mass shift in O, depending on the number of C-termini they contain.
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CEO of Professional Science Editing for Scientists @ prosciediting.com