The genotypic assignment tests of Rannala and Mountain [11] and Pritchard et al. [12] exhibited a high degree of overlap, yielding consistent species assignments for 78.8% of the individuals sampled.
Thus, a stepwise population expansion, inferred by individual assignment test of nDNA, has taken place in association with eco-environmental changes.
Because genome size (GS) values suggested the involvement of E. falcisepalum and E. madsenii in the origin of hybrids in HZ1 (see below), Bayesian assignment tests of this hybrid zone were run with these species (instead of E. rhopalostele) and hybrids indeed showed intermediate q-values in this population.
Haplotype group 3 also contained a mix of individuals from North Fiji and Lau Basin but was not directly connected to haplotype group 2. Assignment tests of individual multi-locus genotypes identified two geographical regions hosting distinct populations of Ifremeria nautilei (STRUCTURE, K = 2; Figure 3): one in Manus Basin and a second occupying North Fiji and Lau Basins.
The assignment tests of STRUCTURE and GENECLASS were done exclusively for the Algarvia animals, the related southern breeds (Alentejana, Garvonesa, Mertolenga and Preta) and the Limousin cattle to determine which Algarvia animals clustered as an independent group, and to detect admixture.
Since the guideline stresses the importance of correct and complete documentation of symptoms and interventions, every module ends with a documentation assignment testing performance of complete documentation by the nurse specialist/participant.
Results of the self-assignment test of 1225 Galápagos marine iguana samples using the approach of Rannala and Mountain [ 48] as implemented in the program GENECLASS2 [ 47].
Although there were seven ambiguous individuals in NWP1 and NWP2 below our assignment criteria, the mean q-values of assignment test for these two clusters were still over 0.99 (Additional file 6, Table S4).
The Bayesian assignment test correctly assigned 20.1% of the individuals to their own source location (22.4 % being the proportion of individuals that could not be assigned to any of the reference populations).
Altogether we found even nearby populations of G. paeba to be significantly differentiated, although assignment tests revealed 24% of individuals as immigrants.
