Moreover, the CVA assignment rates were high for each structure (from 91 to 98% depending to the skeletal unit).
The ETD mass spectra were simplified and the "MS/MS to assignment" conversion rate was increased to 23%.
We also conducted simulations in PASOS to assess (i) whether it was easier to assign an offspring born to wild-born parents than an offspring born to stocked parents and (ii) whether the expected assignment error rate was comparable for the two groups (Appendix S1).
However, when rates of assignment were combined with rates of sequence recovery (i.e., the product of PCR recovery rate with the correct assignment rate), matK was less successful, resolving only 70% of the 143 species compared to 83% for trnH-psbA and 85% for rbcLa (Table 2).
An error rate was calculated by comparing these assignments with the known annotations.
The follow-up Chi-square analysis allowed us to address the first research question from the perspective of case assignment rates, which is the manner in which these instruments are used in clinical practice.
Assignment rates that were based on maximum likelihood with quantitative genotypes were similar to those based on maximum likelihood with perturbed genotypes but, for more than 50% of cases, the two methods resulted in individuals being assigned to different families.
The total clone redundancy and gene assignment error rates were similar for ESTs from the stressed berry library (Library ID 12435).
