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However, the multi-copy nature and intra-genomic variability of the ITS2 [36], [36] often results in the isolation of more than one ITS2 sequence type from an individual Symbiodinium cell, and this interpretational complexity combined with low genetic divergence among ITS2 sequences [e.g. 9] makes the application of this marker in species assignment problematic [16], [37].
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Teachers sent Lipka assignments for problematic students who sat all day in an over-air-conditioned room by the school's main office.
Also, these Iberian samples may have revealed specimens of problematic assignment if hybridization had been generalized.
However, without such an assignment, it is problematic to link a protein to the gene that encodes it.
Because of the problematic assignment of the orientation of ESTs, unambiguous sequences corresponding to the gau region are rare in the database.
Since transcripts that originated from AS often hold high similarity in sequence, it is likely that problematic assignment in gene orthologs would be reported using sequence-based pipelines.
Although Carsonella pangenome contains 54 strain-specific genes (most of them hypothetical proteins), this number could be an artifact due to the problematic assignment to orthologous clusters of several Carsonella genes owing to its high evolutionary divergence.
Non-random assignment can be particularly problematic in observational studies comparing older versus newer pharmaceuticals from similar therapeutic classes due to the phenomenon of channeling.
The ability to retrieve different scaffolds by focusing on a small number of assignment edges promotes a problematic behavior of the optimal assignment method.
Visualizations of course activity intensity may also indicate topics or assignments that are most problematic for students and may also indicate what topics are most difficult or may be most likely to contribute to procrastination, task avoidance, overconfidence, and ultimately student success or failure.
Deadline-based assignments can also prove problematic as college grads are often accustomed to waiting until the last minute.
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