The lower number of tryptic-peptide assignments and the complete lack of EST evidences for ACT1D and ACT2C, however, suggests that these two actins were probably not expressed.
The complete assignment of the backbone amide resonances of both proteins [11], [12] allows for a residue-specific analysis of the proton exchange rates (Fig. S7, S8 and Table S1).
Analysis of the correlations observed in the HSQC, COSY and HMBC spectra led to the complete assignment of the protons and carbons of the molecule, while the relative configuration was proposed on the basis of NOE enhancements.
From H,C heteronuclear NMR correlations (HSQC, g-HMBC) and ROESY spectra of 3, the complete assignment of the H and C signals could be achieved and 3 was delineated to have the constitution of a 3,3-didehydro-4,5,10- 22 H)-tetrahydro-13,3-didehydro-4,5,10- 22eco-4,5-dioxophytoporphyrinate (see Scheme 2).
The assignment of the sequence was almost complete, with the exception of the residues from the flexible loop towards the end of the sequence, which were not visible in the spectra.
This approach ensures to obtain always a fully accurate assignment ensemble at each step of the complete search.
The assignment of the FPQ item codes to the complete item wording can be found in Table 3.
In addition, the IP eLP2-induced structure of iloprost in solution was elucidated through a complete assignment of the 2D 1H NMR spectra for iloprost in the presence of the IP eLP2 segment.
In the current work, from a complete assignment of the IR and Raman spectra and the results obtained from the study of the VCD spectrum of the title compound, the three most stable rotamers have been detected experimentally in the liquid phase for the first time.
