Sentence examples for assignment of strain from inspiring English sources
The 16S rRNA gene sequences analysis thus strongly supports the assignment of strain G18T to the genus Geodermatophilus.
More robust methods for defining lineages, such as single nucleotide polymorphism analysis or whole-genome sequencing, may have allowed for more refined assignment of strain families in this study.
Various phylogenetic tools have been employed in the past for identification of Bacillus, Clostridium and Pseudomonas species based on 16S rRNA genes [ 106- 108], However, here, we have used the conserved marker genes sequences (proteins = 400) supported by ANI for taxonomic assignment of strain RL.
This assay resulted in 100% inclusivity and exclusivity of assignment of strains to the species L. brevis.
There was little difference between technical replicates indicating that there were no sampling effects from amplifying small amounts of starting RNA, giving confidence in the assignment of strain-associated differential expression.
However, assignment of strains CCS02 and 151112C to subclades was incongruent between both analyses.
It also allowed for assignment of strains from Sudan to the 2 described clusters represented by the Arabia_1999_7 and the Central Africa strains.
Using the same concatenated data set for 897 protein-coding sequences, a parsimonious analysis showed same assignment of strains into clades (supplementary fig. S1, Supplementary Material online).
Although we have used the term electronic taxonomy, the assignment of strains to known species and the identification and acceptance of new species cannot realistically be automated as it requires the experience, knowledge and judgement of taxonomists.
The assignment of strains to the sequence clusters was the same, and the only significant difference was a slightly more marked sub-division of the S. mitis group into sub-clusters using minimum evolution.
Thus, a single PCR can provide the assignment of a strain to one these species, reducing the number of PCR reactions and hands-on time for the identification of human isolates of bifidobacteria.
