Samples have been selected in the training set among those showing larger discrepancies in the 2-DE quantification to validate the correct assignment of protein spots.
In conclusion, although the assignment of both spots was highly reliable, we do not believe that the serum derived signals correspond to the LMAN2 protein.
The analysis of the distribution of differentially expressed genes on different chromosomes was done with the help of BRB-ArrayTools Versoftware.2 software, including the assignment of all spotted and differentially expressed genes to specific chromosomes.
Due to limitations of ORF assignment for specific spots of the previously described whole genome microarray platform [ 14], we designed a more targeted and fully validated array to address developmental regulation of the trafficking system in T. brucei.
The reproducibility of the assignment of these 'hot spots' is a critical variable in the analysis of MVD and the success of finding the relevant 'hot spot' depends on the training and experience of the investigator (Vermeulen et al, 2002).
The Biacore 4000 fluidics system allows the assignment of five detection spots to each of four flow cells, enabling the screening of four different compounds per cycle, where one of the targets per flow cell has to be assigned as a reference or preferably left blank.
Two executives have the additional corporate assignment of contacting him when they spot his color fading and suggest that it is time for a touch-up.
Earlier in his military career, the general was responsible for some 92,000 troops ready for assignment to hot spots including Bosnia and Somalia, and he adopted a policy of having a personal interaction with everybody under his command every year.
Taking further into account the correlations between the observed and predicted pI patterns, then an assignment of subunits Hb4, Hb5, and Hb3 to the spots 20, 21 and 22 seems plausible (see Discussion).
Both spots identified as VIP36 reside within these protein tracks confirming the exact assignment of the serum derived signals to the master secretome and to the preparative gel.
Standards for assignment of the "p.g.a".
