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We include protocols for RNA isolation from Saccharomyces cerevisiae, Pseudo-seq library preparation, and data analysis, including descriptions of processing and mapping of sequencing reads, computational identification of sites of pseudouridylation, and assignment of sites to specific pseudouridine synthases.
Bioavailability considerations are one of the tools for a proper assignment of sites potentially and actually at risk as it allows assessing both the extent (hazard) and probability (risk) of adverse effects.
The design was chosen because there was no random assignment of sites to voucher or comparison group.
The design was chosen because there was no random assignment of sites or health facilities to intervention (voucher) or comparison (non-voucher) groups.
The Gibbs sampling procedure enables us to sample in the space of mapping and achieve a reasonable assignment of sites to sequence tags.
A related approach is to allow the data inform the assignment of sites to subsets, and to integrate out the uncertainty in these assignments in a Bayesian framework [ 35].
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Step 4. Based on the current assignments of sites for ambiguous tags obtained from Step 3, the likelihood ratios of all the probable sites are updated.
We compare the various assignments of sites using receiver operating characteristic (ROC) analysis, a commonly used methodology to assess the tradeoff between the sensitivity and specificity of predictors.
A list of class assignment and probabilities for all AA sites is provided in the additional file 2: Class assignment of AA sites.
The results obtained confirm the assignment of adsorption sites previously reported for the adsorption of N2, Ar, and CO2.
The assignment of phosphorylation sites of identified phosphopeptides was performed by the PTM scoring algorithm implemented in MSQuant as described previously [31].
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