Taxonomic assignment of the resulting Roche 454 reads was performed by using the RDP Classifier program version 2.6 with bootstrap value ≥0.5 against the representative sequences of each 97% sequence cluster that was chosen by the UCLUST program.
Crossing clinical and genomic results, and accounting for trial assignment of discordant test results, yielded 8 groups: 1) "C-low/G-low", 2) "C-high/G-high", 3) "C-low/G-high assigned to no CT", 4) "C-low/G-high assigned to CT", 5) "C-high/G-low assigned to no CT", 6) "C-high/G-low assigned to CT", 7) "C-low/G-na", and 8) "C-high/G-na".
Although a major strength of this study included its randomized assignment of treatment, the results presented here must be interpreted cautiously.
For this reason, the performance of the method cannot be effectively evaluated at protein level as the assignment of an exact result over a wrong one is uncertain, whenever a mix of correct and incorrect predictions is recovered for every protein.
Overall our DNAm-based assignment of inactivation status resulted in a lower frequency of XCI than seen with previous assessments of individual genes.
Assignment of GO terms resulted in a total list of 2734 unique GO terms among all 5 age-related disease genes (Fig. 3B).
The exclusion of the VENICE trial and the subsequent assignment of appropriate weights result in an estimate of the incidence of FAEs of 3.2 % (95% CI 1.6 5.6) for single-agent aflibercept.
EM464 and SB146 are fixed for different alleles at microsatellite locus cr52.84, approximately 210 bp and 180 bp respectively, allowing paternity assignment of F1 progeny resulting from crosses between these strains.
Although minor differences were apparent in the assignment of single individuals, the results obtained by Geneland were congruent with the results obtained by Structure (supplementary table S4, Supplementary Material online).
