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This information was combined for each family, resulting in an assignment of reference sequences to families to which no reference was assigned during clustering.
Since the assignment of reference or non-reference allele depends on the reference genome, the assignment per se is not of biological interest.
Since the assignment of reference and non-reference allele depends on the reference genome, whether a SNP is skewed toward reference or non-reference allele per se does not have direct biological meaning.
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7 The problem of selection has been addressed through the use of fixed effects or random assignments of reference groups, such as the practice of randomly assigning first year roommates at some colleges.
Putnam's model-theoretic argument aimed to show that there are very many different assignments of reference to subsentential expressions of our language which make all of our utterances true.
The clustering depicted on this neighbor-joining tree accurately reflects the geographic origins of the reference strains, and is in agreement with previous assignments of the reference strains to distinct population groups [ 18].
To confirm the assignment of HIV subtypes, reference sequences obtained from the Los Alamos Sequence Database and each Gabonese sequence were aligned with the ClustalX program [ 13].
By quantifying all of the template strands in a reaction, this method provides an improvement for the absolute quantification of DNA copy number which could be particularly useful for the assignment of values to reference materials [ 38].
We performed sensitivity analyses by repeating the primary analysis using several different methods of reference creatinine assignment.
Individual samples were compared to the common reference, assignment of fluorescent labels (Cy5 and Cy3) was changed in each hybridization.
This allowed the assignment of 4,367 Atlantic salmon reference genome contigs (corresponding to 57 Mb of sequence) to at least one linkage group (Table 4; Additional file 5).
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