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However, the majority of PRMT substrates are nonhistone proteins, often involved in RNA biochemistry, which complicates the assignment of cellular roles for histone arginine methylation.
Though assignment of cellular location was generally unclear using the PSORT program, Os1bglu2 and Os11bglu36 (Arabidopsis SFR2 homolog) are predicted to localize to the chloroplast, like maize, sorghum, wheat and oat β-glucosidases, though they are not closely related phylogenetically.
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The more represented biological process categories were linked with metabolism, regulation and development, showing a consistent assignment of GO cellular components (data not shown).
Here we expand the ME-model to include (1) a reconstruction of protein translocation pathways, (2) assignment of all cellular proteins to one of four compartments (cytoplasm, inner membrane, periplasm, and outer membrane) and a translocation pathway, (3) experimentally determined translocase catalytic and porin diffusion rates, and (4) a novel membrane constraint that reflects cell morphology.
We did not attempt in this report to indicate explicitly the intracellular orientation of the multiple domains of enzymes in the enhanced pathway representations implemented in the prototype Populus PGDB; our assignment of sub-cellular localization of enzymes in pathway diagrams was intended to portray the site of catalytic activity.
The distribution of unigenes under the biological process category was more uniform, with 19.9% of assignments to cellular process and 12.7% to biosynthesis (Fig. 2C).
Regulation of cell differentiation is an important assignment for cellular engineering.
For eukaryotic cells, stoichiometric reconstruction of the network includes the assignment of reactions to cellular compartments.
Motivation: Experimental spatial proteomics, i.e. the high-throughput assignment of proteins to sub-cellular compartments based on quantitative proteomics data, promises to shed new light on many biological processes given adequate computational tools.
Among the 18 subcategories of cellular components, those assignments were mostly given to cell part and organelle.
Based on the GO assignment of the proteins to their cellular locations, the membrane was proposed to be one of the main foci where pathogenic activity occurs.
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