This study did not involve traffic assignment due to limitations in resources and data.
However, the trial was associated with several limitations; assignment of treatment was not blinded to providers, which may have influenced the results (e.g. knowing that active gel was being used may have allowed the provider to proceed with greater confidence).
Because of this limitation, assignment and sequencing of outbound vehicles at each hub is taken into consideration.
A result that is worse than a random assignment, pointing out the limitations of specialized rules.
Those deemed to be fully employable are sent to a job center for a work assignment; those deemed employable with limitations are sent to vocational rehabilitation programs for specialized job services.
Specifically, it is hard to tackle issues related to the problem structuring, like the existence of constraints on the sequence of decisions, and the time evolution modeling; related to the knowledge-acquisition, like probability and utility assignment; and related to computational limitations, in memory storage and evaluation phases, as well as the explanation of results.
Additional limitations include the assignment of lead exposure based on job titles and of cardiovascular deaths based on death certificates.
Measuring the effectiveness of drugs through observational databases has some limitations, such as assignment of treatment, patient selection bias, and the absence of a washout period [ 19].
However, these systems present some limitations such as assignment of a single vascularity grading and coarse grading scales and are unable to describe blebs with mixed morphology.
Due to limitations of ORF assignment for specific spots of the previously described whole genome microarray platform [ 14], we designed a more targeted and fully validated array to address developmental regulation of the trafficking system in T. brucei.
