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Those genes that have an assigned function have such an assignment due to either an experiment verifying that function or high sequence similarity to an experimentally-confirmed gene.
However, reads having many homologous sequences in the database due to high conservation among microbial families and lineages, lead to a rather "diffuse", but still correct read assignment: due to gene sharing, MEGAN assigns these reads to high-order taxa closer to the root (e.g. at genus level), thus avoiding probable false-positive assignments (MEGAN uses an LCA-based algorithm [13].).
This moral justification seemed more compelling with each new assignment, due to the ever-escalating violence deployed by dealers to deter communities from talking to the police.
Two even bigger cock-ups: the NME offered to send me to New York to exclusively interview Michael Jackson and his brothers in the early 1980s just prior to the mega-success of Thriller, but I had to refuse the assignment due to "health problems".
2. gamma - log odds of differential assignment due to unobserved factors.
This study did not involve traffic assignment due to limitations in resources and data.
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However, the presence of relatively long links in some topologies leads to conflicting channel assignments due to their high interference range.
Additionally, the use of bootstrapping provides a measure of the expected mis-assignment due to local homoplasy [3].
In effect, bootstrapping provides a measure of the expected mis-assignment due to local homoplasy, or of assignment failure due to simple lack of evidence of species monophyly.
We believe that a high percentage of bidirectional reads will be critical for a successful plant barcoding system, given the generally low amount of variation that separates many plant species [8], [30], and the increased danger of mis-assignment due to sequencing error that can be anticipated with incomplete bidirectional reads.
Unfortunately, we were not able to establish full assignments due to poor deuterium to proton back-exchange of the per-deuterated and 13C-15N-labelled 13C-15N-labelled 13C-15N-labelled
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