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Each of the three applications may aid in assigning a function and in classification of binding patterns.
This is achieved by first connecting influencing factors (in white on Figure 8a) to influenced factors (in black on Figure 8a), and assigning a function to each connection.
Insertional mutagenesis, with T-DNA or a transposable element, provides opportunities for assigning a function to a particular DNA sequence and isolating the target gene causing a specific phenotype.
The genetic ablation of these loci, while technically challenging, would be key to assigning a function for these elements.
Current annotation pipelines succeed at assigning a function to 50 80% of the genes (Serres et al., 2004).
As in our previous study of kinases, chemical sensitivity assays proved to be an effective tool in assigning a function for such mutants lacking a morphological phenotype.
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Reverse genomics consists of identifying a specific sequence, either coding or non-coding, and try to assign a function using sequence comparison or functional analysis.
In silico functional annotation based on protein signatures and homologies allowed us to assign a function to 43% of the 11,839 predicted proteins.
You can assign a function to the Shortcut button, which is one of the Zen's handiest features.
Of these 215 genes, an E > 10−10 was used to assign a function for 205 genes, and a value in the range of 10−7 to 10−10 had to be used to assign a function for about 10 genes.
Prior to the release of the sequence, only a few genes were assigned a function each year.
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