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The YA novel was a polarizing assigned read, with some readers embracing the bittersweet romance and others recoiling at the stereotype-laden supporting characters.
Gene expression estimates are included provided the gene has at least one assigned read.
We have highlighted the nodes (showing sum and assigned read numbers) that are used to create Figure 1.
By comparing the mapping position and annotation information, all mapped reads were classified into the corresponding RNA types, and the relative abundance of expression of each RNA type was evaluated based on the assigned read counts.
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The idea is to use k=20 to maximize the number of assigned reads, but only consider high confidence assignments to increase the precision.
However, the high assignment accuracy (correct hits, %TP (Supplementary Information Table S1)) means that practically all assigned reads were correctly classified by MEGAN.
Now, go tackle your assigned reading.
Earlier sections on Picasso, Cézanne and van Gogh note assigned reading in Barr, Greenberg and The Partisan Review.
Though it was assigned reading and passages were discussed in class, I have no memory of my teachers commenting on any biblical parallels.
The book, with its harsh language and scenes, was banned by some schools but soon became assigned reading in many others.
By day, Kirkland's guests discuss assigned reading and lunch at intimate local restaurants, and by evening, they retreat to a cozy hotel room.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com