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aRank: is the rank number calculated using the formula (2.7.1) based on the number of occurrences of the pathways among all the assigned pathways.
When analyzing dominant KEGG pathways assigned to the unigenes expressed in each tissue, the most commonly assigned KEGG pathways generally followed the most commonly assigned pathways for T. bernacchii as a whole, with a few exceptions.
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TargetP reliability of the assigned pathway was, however, highly variable between sequences.
* Ratio of altered genes in bone marrow adipocytes to assigned pathway.
On the other hand, 138 genes out of total 398 genes were identified and 66 genes were assigned pathway during the post freezing recovery period.
The unbiased approach we have used to select candidate genes has also highlighted a gene whose currently assigned pathway (circadian rhythm) does not overtly relate to mucosal inflammation.
The list of resulting protein ids with KO ids was processed to assign pathways and EC numbers using perl scripts.
To find a connection between the differentially expressed genes bioinformatic tools (Ingenuity Pathway Analysis; Ingenuity systems Inc., CA, USA, and PathwayAssist; Stratagene, CA, USA) and literature studies were used to assign pathways for these genes.
This web-based analysis tool enables the detection of enrichment of gene ontology (GO) terms in a set of genes and uses the Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation to assign pathways that are significantly affected.
Each ranked list was imported into the Ingenuity Pathways Analysis 6.3 (IPA, from Ingenuity® Systems, www.ingenuity.com) which incorporates an extensive literature-derived knowledge base from which to assign pathway affiliation.
This program assigns pathway significance based upon the number of genes represented within a pathway and the direction of change.
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